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Fig. 5 | Biological Research

Fig. 5

From: Endocannabinoid system upregulates the enrichment and differentiation of human iPSC- derived spermatogonial stem cells via CB2R agonism

Fig. 5

hiPSCs carry cannabinoid receptors. A hiPSCs express CB1R, CB2R, TRPV1 and GPR55 relative to hDF. B Homogenous high percentage of CB1R, CB2R, TRPV1 and GPR55 distribution on hiPSCs by FCM. C CB1R, CB2R, TRPV1 and GPR55 immune distribution in hiPSCs by FCM. D Representative diagram for co-distribution of CB1R, CB2R, TRPV1 and GPR55 and stemness marker TRA-1-60 on hiPSCs by FCM. E Nucleo-cytoplasmic (arrow) labeling of CB1R, CB2R, TRPV1 and GPR55 on hiPSCs by IF. Scale bar is 50 μm. F Positive correlation for CB1R, CB2R, TRPV1 and GPR55 mRNA expression by qPCR and immunolabeling by FCM. *p < 0.05

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