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Fig. 2 | Biological Research

Fig. 2

From: Endocannabinoid system upregulates the enrichment and differentiation of human iPSC- derived spermatogonial stem cells via CB2R agonism

Fig. 2

hDFs are reprogrammed to hiPSCs. A hiPSCs highly express OCT4, SOX2 and NANOG relative to hDFs. B. carry OCT4 + SSEA4, SOX2 + TRA-1-60 and NANOG by FCM. D hiPSC colonies (*) present clear and well-defined borders with cytoplasmic OCT4, SOX2, NANOG, SSEA4 and TRA-1-60 immunolabeling by IF. Scale bar is 200 μm, scale bar is 50 μm inlets. E Positive correlation for OCT4, SOX2, NANOG, SSEA4 and TRA-1-60 expressions by qPCR and immunolabeling by FCM. * p < 0.05

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Biological Research

ISSN: 0717-6287

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