Fig. 6
Immunocytochemistry analysis of CD133 marker expression in malignant cell lines in 24 h and 72 h after EP +/- Ca2+ application. (A) LoVo (sensitive human colorectal adenocarcinoma cell line) and (B) LoVo Dx (doxorubicin-resistant human colorectal adenocarcinoma cell line) treated with 2 mM of calcium ions and electroporation (1.2 kV/cm, 8 pulses, 100 us, 1 Hz; 37.5 kV/cm and 50 kV/cm, 200 pulses 10 ns, 10 Hz). Scale bar, 20 μm; (C) graphs present the percent of CD133-positive cells for each tested parameter 24 and 72 h after therapy. n ≤ 4; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS-not significant (comparison to control)