Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.

You are viewing the site in preview mode

Skip to main content
Fig. 5 | Biological Research

Fig. 5

From: Calcium electroporation induces stress response through upregulation of HSP27, HSP70, aspartate β-hydroxylase, and CD133 in human colon cancer cells

Fig. 5

Immunofluorescence visualization of heat shock protein 70 (HSP70) in malignant cell lines after EP +/- Ca2+ application. LoVo (sensitive human colorectal adenocarcinoma cell line) and LoVo Dx (doxorubicin-resistant human colorectal adenocarcinoma cell line) treated with 2 mM of calcium ions and electroporation (1.2 kV/cm, 8 pulses, 100 us, 1 Hz; 37.5 kV/cm and 50 kV/cm, 200 pulses 10 ns, 10 Hz). Right panel: graphs present HSP70 signal intensity for each tested parameter 24 and 72 h after therapy; n = 4–5. Left panel: CLSM images of HSP70 signal changes (green) visualized 24 h after CaEP application; 250 μm; 60.0X oil immersion objective; NA: 1.35. n ≤ 3; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, NS-not significant (comparison to control)

Back to article page

Biological Research

ISSN: 0717-6287

Contact us