Fig. 1

The effect of CaCl₂ and electroporation on cell viability assessed by MTT and SRB assays. (A) Cell viability of LoVo, LoVo Dx, and Hs378st.int after 24 h of treatment with electroporation (1.2 kV/cm, 8 pulses, 100 us, 1 Hz; 37.5 kV/cm and 50 kV/cm, 200 pulses, 10 ns, 10 Hz) with or without 2 mM Ca²⁺, measured by MTS assay. (B) The MTS assay assessed the cell viability of the same cell lines under the same conditions after 72 h. (C) Cell viability of LoVo, LoVo Dx, and Hs378st.int cell lines after 24-hour exposure to increasing concentrations of CaCl₂, assessed using the MTS assay. (D) The viability of LoVo and LoVo Dx cells after 72 h of treatment with electroporation (1.2 kV/cm, 8 pulses, 100 us, 1 Hz; 37.5 kV/cm and 50 kV/cm, 200 pulses, 10 ns, 10 Hz) with or without 2 mM Ca²⁺, evaluated using the SRB assay. Results are expressed as a percentage of control cell viability. Mean ± SD, n ≤ 6; *p < 0.05, **p < 0.01, ***p < 0.001, ***p < 0.0001(comparison to control)