Fig. 3
hslJ promoter sequence and OxyR-binding assay. (A) In silico analysis of hslJ promoter region. A putative OxyR consensus sequence was located − 95 bp upstream of the ATG initiation codon; the − 35 and − 10 consensus sequences resembling those recognized by RpoD were detected. The putative ribosome binding site (RBS) is also shown. (B) Graphical representation of the consensus sequence generated with WebLogo server (http://weblogo.berkeley.edu), based on the multiple alignment showed in Table S2. (C) Increasing amounts of purified OxyR were incubated with the hslJ promoter oligo (PhslJ). OxyR binding activity was measured colorimetrically as the amount of the immobilized oligo-protein complex recognized by the primary anti-6xHis, and HRP-conjugated secondary antibodies