Fig. 2

Impact of the hslJ mutation on cell survival under oxidative conditions. (A) Quantification of the survival rates of the indicated strains to 5 mM of H₂O₂ in the presence or absence of IMP (4 µg/ml). (B) Intracellular survival rates of WT, hslJ mutant, and hslJ(pAhslJ) in RAW 264.7 TIB-71™ macrophages infected at a MOI of 100, 10 min after the addition of colistin sulphate (10 µg/ml). (C) Representative images of a parallel set of infected cell plates fixed, and stained with LysoTracker Red DND-99 and DAPI. (D) hslJ mRNA expression levels in the presence and absence of 5 mM H₂O₂ and 4 µg/ml IMP, assessed by qRT-PCR. Data are shown as means + SDs (n = 6) from 3 independent experiments. Statistical significance was evaluated by one-way ANOVA