Fig. 1
Surface motility, biofilm formation, and CSH of the hslJ mutant. (A) The WT, hslJ mutant, and the complemented strain hslJ(pAhslJ) were exponentially cultured in the presence and absence of IMP. CFU/ml were enumerated after 2 h. (B) Representative image of surface motility of the indicated strains assayed on semisolid (0.25%) LB agar plates. (C) Quantification of biofilm formed by the WT, hslJ mutant, and the complemented strain hslJ(pAhslJ) measured by crystal violet stain in a 96-well culture plate in the presence and absence of IMP. Results are reported as the OD570/OD600 ratio to normalize the amount of biofilm formed to the total bacterial content. (D) Quantification of the amount of Congo red retained by each strain. Results are reported as the OD490/OD600 ratio to normalize the amount of Congo red dye bound to the total bacterial content. (E) Representative images of SAT. Data are shown as means + SDs (n = 6). Statistical significance was evaluated by one-way ANOVA