Table 1 List of PCR primers used to validate mutations by Sanger sequencing
Gene | Primer forward | Sequence 5’->3’ | Primer reverse | Sequence 5’->3’ | PCR product size |
|---|---|---|---|---|---|
BRCA1 | rs80357208-F | AGGATGAAGAGCTTCCCTGC | rs80357208-R | GACCAACTCCCTGGCTTTCA | 340 bp |
rs80357505-F | TGACTCACATGATGGGGAGTC | rs80357505-R | ACCATTCTGCTCCGTTTGGT | 440 bp | |
BRCA2 | rs11571658-F | ACGAACATTCAGACCAGCTCA | rs11571658-R | GGTGAAGCCTGTTCTTTTCCC | 505 bp |
PALB2 | rs876659571-F | ACTGAGTCCTAAACGCATGGA | rs876659571-R | TGTTTATGCAGCTCCTGGCA | 450 bp |
TP53 | rs1131691022-F | AGGTACTTGAAGTGCAGTTTCT | rs1131691022-R | TGTGGTTATAGGATTCAACCGGA | 420 bp |