Table 1 Details of immunohistochemical techniques
Primary antibodies | Antigen retrieval | Dilution | Time and incubation conditions | Visualization kit | Chromogen |
|---|---|---|---|---|---|
Rabbit antibodies to S-100 (Sigma-Aldrich Cat# S2644, Darmstadt, Germany, RRID: AB_477501) | No | 1:300 | 18–20 h at 8 C˚ | HRP-F(ab’)2α rabbit IgG, 1:500 (Thermo Fisher Scientific Inc., Kalamazoo, MI, USA Cat# A24531, RRID: AB_2535999) | DAB* |
Rabbit polyclonal antibodies to neuron-specific β-III tubulin (Abcam Cat# ab229590, Cambridge, CB2 0AX, UK, RRID: AB_2827733) | No | 1:1000 1:2000 | 1 h at room t0 | Ultra Vision LP Detection System (Thermo Fisher Scientific Inc., Kalamazoo, MI, USA) | DAB |
Mouse monoclonal antibody to NeuN clone A60 (Millipore Cat# MAB377, Darmstadt, Germany, RRID: AB_2298772) | Yes | 1:100 | 1 h at room t0 | Ultra Vision LP Detection System (Thermo Fisher Scientific Inc., Kalamazoo, MI, USA) | DAB |
Mouse monoclonal antibody to reelin clone 142 (Millipore Cat# MAB5366, Darmstadt, Germany, RRID: AB_2285132) | No | 1:250 | 1 h at room t0 | Ultra Vision Plus Detection System (Thermo Fisher Scientific Inc., Kalamazoo, MI, USA) | DAB |