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Fig. 7 | Biological Research

Fig. 7

From: Depression like-behavior and memory loss induced by methylglyoxal is associated with tryptophan depletion and oxidative stress: a new in vivo model of neurodegeneration

Fig. 7

Methylglyoxal (MGO) down-regulated Nrf-2/Ho-1, Trx, and Sirt family proteins expression and upregulated GR expression in mice. (A) The expressed levels of Nrf-2, HO-1, Trx, TXNRD1, GR, and GAPDH by western blotting assay. The densitometry graph of each band. (B) Immunostaining detection of GR. The densitometry graph of each figure. Scale bar: 50 μm (20× magnification). (C, D) NAD+ and CAT activity by colorimetric assay kit. (E) The expressed levels of Sirt-1, Sirt-2, Sirt-3, Sirt-4, Sirt-5, Sirt-7, and GAPDH by western blotting assay. (F) Molecular docking analysis between MGO and Sirt-3/Sirt-5 using AutoDock Vina software. All data presented as mean ± SEM (n = 3). #p < 0.05 and ##p < 0.01, and ###p < 0.001 vs. control group (CON). CON, control group. MGO, 65 mg/kg of MGO rectal injection group

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Biological Research

ISSN: 0717-6287

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