Fig. 5

Photomicrograph of the longitudinal section of the midgut of A. mellifera workers after oral exposure to imidacloprid. A, B and C = control; D, E and F = commercial formulation IMD; G, H and I = active ingredient IMD. Ep = epithelium of midgut; m = musculature; L = lumen; Pm = peritrophic membrane; cm = circular musculature; Im = longitudinal musculature; Dc = digestive cell; bl = basal lamina; rc = regenerated cells; n = nucleus; c = cytoplasm; b = striated border; Ep* = deformed epithelium; Dc* = release of digestive cells to lumen; Pm* = degenerated peritrophic membrane; Mt = Malpighian tubule; * = vacuolated digestive cells; t = traqueiolas; → = cytoplasm protrusion; ↔ = loosening of the longitudinal musculature and separation from the circular musculature; ⊳ = intercellular spaces. Staning: Hematoxylin–eosin. Scale: A = 100 µm; B-l = 20 µm