Fig. 5

Knockout of Ezh1/2 interferes with the expression of many functional genes in fetal mouse ovaries. Gene transcription in embryo ovaries were sequenced on an Illumina HiSeq 2500 with 125 bp single end sequencing and gene expression data were analyzed by using DAVID (the Database for Annotation, Visualization, and Integrated Discovery, [https://david.ncifcrf.gov]). (A) Volcano plot of differentially expressed genes in WT and dKO ovary tissues on 14.5dpc + 3 days as determined by RNA-seq. Blue or red dots represent genes that are differentially expressed (|log2FC|>=1, Qvalue < = 0.05) between WT and dKO group. Red dots indicate upregulated genes, and green dots indicate down-regulated genes. Genes not significantly differentially expression (no DEGs) are shown in gray. The FC in log2FC is fold change, which represents the ratio of the expression between the two samples (groups). Log10 (Expression of WT/dKO): the normalized expression value is taken as the log, usually using log10. (B) Heatmap of physiological function associated genes differentially regulated between WT and dKO ovary tissues on 14.5dpc + 3 days. Heatmap of the clustering of the different expressed genes. Green indicates lower expression, red indicates higher. FPKM of each gene from different samples was normalized to Z-score. (C-F) The relative expression level of genes in fetal ovaries treated with lentivirus by RT-qPCR. Gapdh is a reference gene. The values in each group were averaged and normalized with that of WT group. The experiments were repeated at least 3 times (N = 3). Data were presented as the mean ± S.D; The asterisk (*) denotes a statistically significant difference between the WT and dKO groups. *P < 0.05, **P < 0.01 (t-test)