Fig. 4

Ezh1/2 function deficiency results in abnormal expression of genes involved in the initiation of DSBs. (A-B) The protein level of hormad1 in fetal ovaries treated with UNC1999 or lentivirus. GAPDH and ERK were used as an internal control. Quantitative analysis of Hormad1 protein level normalized by GAPDH protein. Every group had 20 ovaries. (C-J) The relative expression levels of Hormad1, Mre11, Rad50, and Nbs1 mRNA in fetal ovaries treated with UNC1999 or lentivirus (dKO) by RT-qPCR. Gapdh is a reference gene. The data were normalized with that WT at 14.5 dpc + 0 days. The experiments were repeated at least 3 times (N = 3). Data were presented as mean ± S.D. The asterisk (*) denotes a statistically significant difference between the vehicle (or WT) and treatment groups. *P < 0.05, **P < 0.01 (t-test)