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Fig. 2 | Biological Research

Fig. 2

From: Phenotyping of a new yeast mapping population reveals differences in the activation of the TORC1 signalling pathway between wild and domesticated yeast strains

Fig. 2

Overview of the method for indirect monitoring of TORC1 activation using the TOMAN-G population. The TOMAN-G population was generated by transforming 274 yeast strains that were originally part of the “1002 Yeast Genomes Project” population with the pTOMAN-G plasmid. These strains were evaluated in nitrogen upshift experiments by growing them in 96-well plates with YMM + Pro medium and adding glutamine (Gln) as a nitrogen source when the strains reached OD600 ~ 0.8. The luminescence (Lum) was recorded after addition of glutamine every 10 min. [Created in BioRender.com]

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Biological Research

ISSN: 0717-6287

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