Fig. 1

Construction of the pTOMAN-G plasmid. The main features for the expression and replication of the plasmid in yeast are shown. The RPL26A promoter (P_RPL26A), a downstream target of TORC1, was used to control the expression of the luciferase (Luc) reporter gene. The genetic construct was assembled in the centromeric pRS316 plasmid, including the CYC1 terminator (CYC1_ter) and the hygromycin cassette (HphMx) as a selectable marker. It also kept the original URA3 gene from the pRS316 plasmid as another selectable marker. This plasmid was used to transform a subpopulation of diploid-euploid strains derived from the “1002 Yeast Genomes Project” population