Fig. 3

Ptprd+/- or Ptprd-/- mice show an increase of GABAergic neurons in the mPFC and somatosensory cortex at 3 months. (A-H) Coronal sections of the mPFC or somatosensory cortex of Ptprd+/+ (PTPRD-WT) and Ptprd-/- (PTPRD-KO) 3-month-old animals were immunostained against parvalbumin (A, E) or somatostatin (C, G). Relative total parvalbumin-positive cells in the mPFC (for B, PTPRD-WT, n = 4; PTPRD-HET, n = 3; PTPRD-KO, n = 5) or somatosensory cortex (for F, PTPRD-WT, n = 4; PTPRD-HET, n = 5; PTPRD-KO, n = 3) of Ptprd+/+, Ptprd+/- (PTPRD-HET), and Ptprd-/- 3-month-old mice. Relative total of somatostatin-positive cells (arrows head) in the mPFC (for D, PTPRD-WT, n = 4; PTPRD-HET, n = 3; PTPRD-KO, n = 4) or somatosensory cortex (for H, PTPRD-WT, n = 3; PTPRD-HET, n = 4; PTPRD-KO, n = 4) of Ptprd+/+, Ptprd+/- and Ptprd-/- 3-month-old mice. In (A) and (C), the dotted line shows mPFC and specific cortical layers. (I, J) Parvalbumin (I) or Somatostatin (J) positive neurons were quantified per cortical layers II/III, V and VI in the mPFC. (K, L) Parvalbumin (K) or Somatostatin (L) positive neurons were quantified per cortical layers II/III, IV, V and VI in the somatosensory cortex. mPFC = medial prefrontal cortex. SSC = somatosensory cortex. PV = parvalbumin. SOM = somatostatin. WM = white matter. In all the images, scale bars represent 200 μm, and arrows show positive cells. *p < 0.05; **p < 0.01; ***p < 0.001. In all graphs, the error bars denote SEMs