Fig. 2

Excitatory synaptic transmission in layer 2/3 of the mPFC is disrupted in the Ptprd-/- mice. (A) Sample traces (left) and quantitative analysis (right) of sEPSC activity recorded from layer 2/3 pyramidal neurons in the mPFC in Ptprd+/+ (PTPRD-WT), Ptprd+/- (PTPRD-HET), and Ptprd-/- (PTPRD-KO) mice. A significant increase in the frequency but not in the amplitude of sEPSC in PTPRD-KO was detected. PTPRD-WT, n = 4 animals, 10 cells; PTPRD-HET, n = 5 animals, 11 cells; PTPRD-KO, n = 8 animals, 15 cells. **p < 0.01. (B) Miniature excitatory synaptic current (mEPSC) also shows an increase in the frequency but not in the amplitude in Ptprd-/- compared to Ptprd+/+ and Ptprd+/- mice. PTPRD-WT, n = 5 animals, 8 cells; PTPRD-HET, n = 5 animals, 8 cells; PTPRD-KO, n = 6 animals, 11 cells. *p < 0.05. (C) Input/output function measured as the EPSP amplitude as a function of stimulus intensity also is increased in Ptprd-/- compared to Ptprd+/+ and Ptprd+/- mice. PTPRD-WT, n = 3 animals, 6 cells; PTPRD-HET, n = 5 animals, 9 cells; PTPRD-KO, n = 5 animals, 7 cells. (D) Paired-pulse responses superimposed after subtraction of the first pulse at 10, 30, 70, 100, and 300 ms interstimulus intervals (ISI) are similar between genotyping. PTPRD-WT, n = 4 animals, 6 cells; PTPRD-HET, n = 6 animals, 8 cells; PTPRD-KO, n = 5 animals, 8 cells. (E) Short-term synaptic responses (top) and normalized summary data (bottom) evoked by a burst of 25 stimuli at 14 Hz are also similar between genotyping. PTPRD-WT, n = 3 animals, 6 cells; PTPRD-HET, n = 4 animals, 8 cells; PTPRD-KO, n = 4 animals, 9 cells. (F) AMPA/NMDA ratio shows no significant differences between genotyping. PTPRD-WT, n = 3 animals, 6 cells; PTPRD-HET, n = 3 animals, 8 cells; PTPRD-KO, n = 5 animals, 14 cells. In all graphs, the error bars denote SEMs