Fig. 1

Adult Ptprd+/- or Ptprd-/- mice show an increase of glutamatergic neurons in the mPFC and somatosensory cortex. (A-H) Coronal sections of mPFC (A, C) or somatosensory cortex (E, G) of Ptprd+/+ (PTPRD-WT) and Ptprd-/- (PTPRD-KO) 3-month-old animals were immunostained against Tbr1 (A, E) or Satb2 (C, G). Relative total Tbr1-positive cells in the mPFC (for B, PTPRD-WT, n = 3; PTPRD-HET, n = 4; PTPRD-KO, n = 4) or somatosensory cortex (for F, PTPRD-WT, n = 3; PTPRD-HET, n = 5; PTPRD-KO, n = 5) of Ptprd+/+, Ptprd+/- (PTPRD-HET), and Ptprd-/- 3-month-old mice were quantified. Relative total Satb2-positive cells in the mPFC (for D, PTPRD-WT, n = 3; PTPRD-HET, n = 3; PTPRD-KO, n = 4) or somatosensory cortex (for H, PTPRD-WT, n = 3; PTPRD-HET, n = 4; PTPRD-KO, n = 5) of Ptprd+/+, Ptprd+/-, and Ptprd-/- 3-month-old mice were quantified. In (A) and (C), the dotted line shows mPFC and specific cortical layers. (I, J) Tbr1 (I) or Satb2 (J) positive neurons were quantified per cortical layers II/III, V and VI in the mPFC. (K, L) Tbr1 (K) or Satb2 (L) positive neurons were quantified per cortical layers II/III, IV, V and VI in the somatosensory cortex. mPFC = medial prefrontal cortex. SSC = somatosensory cortex. WM = white matter. *p < 0.05; **p < 0.01; ***p < 0.001. In all the images, scale bars represent 200 μm. In all graphs, the error bars denote SEMs